› 4fxi

X-ray diffraction
1.8Å resolution

Crystal structure of the isolated E. coli RelE toxin, P21 form

Released:
DOI: 10.2210/pdb4fxi/pdb
PDB entry 4fxi coloured by chain, front view.
PDB entry 4fxi coloured by chain, side view.
PDB entry 4fxi coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The crystal structure of the intact E. coli RelBE toxin-antitoxin complex provides the structural basis for conditional cooperativity.
Bøggild A, Sofos N, Andersen KR, Feddersen A, Easter AD, Passmore LA, Brodersen DE
Structure 20 1641-8 (2012)
PMID: 22981948

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-142866 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mRNA interferase toxin RelE Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 95 amino acids
Theoretical weight: 11.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0C077 (Residues: 1-95; Coverage: 100%)
Gene names: JW1555, b1563, relE
Sequence domains: ParE toxin of type II toxin-antitoxin system, parDE
Structure domains: RelE-like

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
1 modified residue:
Ligand CME 3 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 42.57Å b: 61.14Å c: 70.35Å
α: 90° β: 102.93° γ: 90°
R-values:
R R work R free
0.186 0.184 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Toxin-antitoxin systems in bacterial growth arrest and persistence.
Page et al. (2016)
15 more

7 mentions without citation

Wake me when it's over - Bacterial toxin-antitoxin proteins and induced dormancy.
Coussens et al. (2016)
6 more