› 4fxp

X-ray diffraction
1.95Å resolution

Crystal structure of adenosine 5'-phosphosulfate kinase from Arabidopsis thaliana in Complex with Sulfate and APS

Released:
DOI: 10.2210/pdb4fxp/pdb
PDB entry 4fxp coloured by chain, front view.
PDB entry 4fxp coloured by chain, side view.
PDB entry 4fxp coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Nucleotide binding site communication in Arabidopsis thaliana adenosine 5'-phosphosulfate kinase.
Ravilious GE, Jez JM
J Biol Chem 287 30385-94 (2012)
PMID: 22810229

Function and Biology Details

Reaction catalysed: 
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174988 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylyl-sulfate kinase 1, chloroplastic Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 200 amino acids
Theoretical weight: 22.1 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q43295 (Residues: 77-276; Coverage: 73%)
Gene names: AKN1, APK1, At2g14750, F26C24.11, T6B13.1
Sequence domains: Adenylylsulphate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ADX 3 x ADX
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 120.948Å b: 92.348Å c: 73.183Å
α: 90° β: 113.52° γ: 90°
R-values:
R R work R free
0.167 0.166 0.189
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Adenosine-5'-phosphosulfate--a multifaceted modulator of bifunctional 3'-phospho-adenosine-5'-phosphosulfate synthases and related enzymes.
Mueller et al. (2013)
2 more

4 mentions without citation

Nucleotide binding site communication in Arabidopsis thaliana adenosine 5'-phosphosulfate kinase.
Ravilious et al. (2012)
3 more