› 4fxs

X-ray diffraction
2.24Å resolution

Inosine 5'-monophosphate dehydrogenase from Vibrio cholerae complexed with IMP and mycophenolic acid

Released:
DOI: 10.2210/pdb4fxs/pdb
PDB entry 4fxs coloured by chain, front view.
PDB entry 4fxs coloured by chain, side view.
PDB entry 4fxs coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Entry authors: Osipiuk J, Maltseva N, Makowska-Grzyska M, Jedrzejczak R, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192099 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine-5'-monophosphate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 496 amino acids
Theoretical weight: 53.61 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KTW3 (Residues: 1-489; Coverage: 100%)
Gene names: VC_0767, guaB
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand IMP 1 x IMP
Ligand MOA 1 x MOA
Ligand K 1 x K
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I422
Unit cell:
a: 111.96Å b: 111.96Å c: 188.994Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.232
Expression system: Escherichia coli

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