› 4fxx

X-ray diffraction
2.48Å resolution

Structure of SF1 coiled-coil domain

Released:
DOI: 10.2210/pdb4fxx/pdb
PDB entry 4fxx coloured by chain, front view.
PDB entry 4fxx coloured by chain, side view.
PDB entry 4fxx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex.
Wang W, Maucuer A, Gupta A, Manceau V, Thickman KR, Bauer WJ, Kennedy SD, Wedekind JE, Green MR, Kielkopf CL
Structure 21 197-208 (2013)
PMID: 23273425

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-172354 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Splicing factor 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 112 amino acids
Theoretical weight: 12.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15637 (Residues: 26-132; Coverage: 17%)
Gene names: SF1, ZFM1, ZNF162
Sequence domains: Splicing factor 1 helix-hairpin domain
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
Ligand IMD 8 x IMD
Ligand MLI 2 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 96.03Å b: 37.97Å c: 144.68Å
α: 90° β: 107.38° γ: 90°
R-values:
R R work R free
0.239 0.236 0.27
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Unmasking the U2AF homology motif family: a bona fide protein-protein interaction motif in disguise.
Loerch et al. (2016)
5 more

2 mentions without citation

Structural basis for regulation of RNA-binding proteins by phosphorylation.
Thapar R. (2015)
1 more