› 4fyf

X-ray diffraction
2.42Å resolution

Structural basis for substrate recognition by a novel Legionella phosphoinositide phosphatase

Released:
DOI: 10.2210/pdb4fyf/pdb
PDB entry 4fyf coloured by chain, front view.
PDB entry 4fyf coloured by chain, side view.
PDB entry 4fyf coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Primary publication:
Structural basis for substrate recognition by a unique Legionella phosphoinositide phosphatase.
Hsu F, Zhu W, Brennan L, Tao L, Luo ZQ, Mao Y
Proc Natl Acad Sci U S A 109 13567-72 (2012)
PMID: 22872863

Function and Biology Details

Reaction catalysed: 
Phosphatidylinositol 3,4,5-trisphosphate + H(2)O = phosphatidylinositol 4,5-bisphosphate + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178586 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SidF, inhibitor of growth family, member 3 Chain: A
Molecule details ›
Chain: A
Length: 761 amino acids
Theoretical weight: 86.19 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5ZSD5 (Residues: 1-760; Coverage: 83%)
Gene names: lpg2584, sidF
Sequence domains: Phosphoinositide phosphatase

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand HG 3 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 71.835Å b: 115.709Å c: 125.114Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.211 0.27
Expression system: Escherichia coli BL21

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Citations

24 review citations

Type IV secretion in Gram-negative and Gram-positive bacteria.
Grohmann et al. (2018)
23 more

1 mention without citation

Structural basis for substrate recognition by a unique Legionella phosphoinositide phosphatase.
Hsu et al. (2012)