› 4fyh

X-ray diffraction
2.44Å resolution

Crystal structure of rcl with phospho-triciribine

Released:
DOI: 10.2210/pdb4fyh/pdb
PDB entry 4fyh coloured by chain, front view.
PDB entry 4fyh coloured by chain, side view.
PDB entry 4fyh coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structure of the oncoprotein Rcl bound to three nucleotide analogues.
Padilla A, Amiable C, Pochet S, Kaminski PA, Labesse G
Acta Crystallogr D Biol Crystallogr 69 247-55 (2013)
PMID: 23385460

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128714 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-hydroxymethyl-dUMP N-hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 152 amino acids
Theoretical weight: 17.17 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O35820 (Residues: 11-151; Coverage: 87%)
Gene names: Dnph1, Rcl
Sequence domains: Nucleoside 2-deoxyribosyltransferase
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand TR5 4 x TR5
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 92.953Å b: 119.002Å c: 57.769Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.246
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The druggability of intracellular nucleotide-degrading enzymes.
Rampazzo et al. (2016)
3 other articles

1 mention without citation

Survey of phosphorylation near drug binding sites in the Protein Data Bank (PDB) and their effects.
Smith et al. (2015)