› 4fyk

X-ray diffraction
1.79Å resolution

Crystal structure of rcl with 5'-phiosphorothioate-adenosine

Released:
DOI: 10.2210/pdb4fyk/pdb
PDB entry 4fyk coloured by chain, front view.
PDB entry 4fyk coloured by chain, side view.
PDB entry 4fyk coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structure of the oncoprotein Rcl bound to three nucleotide analogues.
Padilla A, Amiable C, Pochet S, Kaminski PA, Labesse G
Acta Crystallogr D Biol Crystallogr 69 247-55 (2013)
PMID: 23385460

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128714 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-hydroxymethyl-dUMP N-hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 152 amino acids
Theoretical weight: 17.17 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O35820 (Residues: 11-151; Coverage: 87%)
Gene names: Dnph1, Rcl
Sequence domains: Nucleoside 2-deoxyribosyltransferase
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand SRA 4 x SRA
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21212
Unit cell:
a: 86.38Å b: 128.772Å c: 57.506Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.194 0.222
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The druggability of intracellular nucleotide-degrading enzymes.
Rampazzo et al. (2016)
3 other articles

1 mention without citation

Dynamics of GreB-RNA polymerase interaction allow a proofreading accessory protein to patrol for transcription complexes needing rescue.
Tetone et al. (2017)