› 4g0x

X-ray diffraction
1.35Å resolution

Crystal Structure of Arabidopsis thaliana AGO1 MID domain

Released:
DOI: 10.2210/pdb4g0x/pdb
PDB entry 4g0x coloured by chain, front view.
PDB entry 4g0x coloured by chain, side view.
PDB entry 4g0x coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Arabidopsis Argonaute MID domains use their nucleotide specificity loop to sort small RNAs.
Frank F, Hauver J, Sonenberg N, Nagar B
EMBO J 31 3588-95 (2012)
PMID: 22850669

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126444 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein argonaute 1 Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.67 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O04379 (Residues: 593-738; Coverage: 14%)
Gene names: AGO1, At1g48410, F11A17.3, T1N15.2
Sequence domains: Mid domain of argonaute
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P212121
Unit cell:
a: 37.25Å b: 60.973Å c: 67.809Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.179
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Argonaute proteins: functional insights and emerging roles.
Meister G. (2013)
17 more

1 mention without citation

Arabidopsis Argonaute MID domains use their nucleotide specificity loop to sort small RNAs.
Frank et al. (2012)