› 4g1v

X-ray diffraction
2.1Å resolution

X-ray structure of yeast flavohemoglobin

Released:
DOI: 10.2210/pdb4g1v/pdb
PDB entry 4g1v coloured by chain, front view.
PDB entry 4g1v coloured by chain, side view.
PDB entry 4g1v coloured by chain, top view.
Source organism: Saccharomyces cerevisiae YJM789
Primary publication:
Active site analysis of yeast flavohemoglobin based on its structure with a small ligand or econazole.
El Hammi E, Warkentin E, Demmer U, Marzouki NM, Ermler U, Baciou L
FEBS J 279 4565-75 (2012)
PMID: 23095020

Function and Biology Details

Reaction catalysed: 
2 nitric oxide + 2 O(2) + NAD(P)H = 2 nitrate + NAD(P)(+) + H(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-108187 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
nitric oxide dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 399 amino acids
Theoretical weight: 44.72 KDa
Source organism: Saccharomyces cerevisiae YJM789
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A6ZUP2 (Residues: 1-399; Coverage: 100%)
Gene names: SCY_2125, YHB1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand K 1 x K
Ligand NO2 1 x NO2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: R32
Unit cell:
a: 172.629Å b: 172.629Å c: 82.294Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.177 0.247
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Flavohaemoglobin: the pre-eminent nitric oxide-detoxifying machine of microorganisms.
Poole RK. (2020)
8 other articles

7 mentions without citation

Direct Electron Transfer of Enzymes Facilitated by Cytochromes.
Ma et al. (2019)
6 more