› 4g23

X-ray diffraction
1.98Å resolution

Crystal Structure of proteinaceous RNase P 1 (PRORP1) from A. thaliana with Mn

Released:
DOI: 10.2210/pdb4g23/pdb
PDB entry 4g23 coloured by chain, front view.
PDB entry 4g23 coloured by chain, side view.
PDB entry 4g23 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Mitochondrial ribonuclease P structure provides insight into the evolution of catalytic strategies for precursor-tRNA 5' processing.
Howard MJ, Lim WH, Fierke CA, Koutmos M
Proc Natl Acad Sci U S A 109 16149-54 (2012)
PMID: 22991464

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179276 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteinaceous RNase P 1, chloroplastic/mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 501 amino acids
Theoretical weight: 57.32 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q66GI4 (Residues: 77-572; Coverage: 87%)
Gene names: At2g32230, F22D22.2, PRORP1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 41.854Å b: 111.863Å c: 139.859Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.22
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Pentatricopeptide repeat proteins in plants.
Barkan et al. (2014)
16 more

8 mentions without citation

Structural conservation of the PIN domain active site across all domains of life.
Senissar et al. (2017)
7 more