› 4g2d

X-ray diffraction
2.7Å resolution

Crystal structure of the hyperthermophilic Sulfolobus islandicus PLL SisLac

Released:
DOI: 10.2210/pdb4g2d/pdb
PDB entry 4g2d coloured by chain, front view.
PDB entry 4g2d coloured by chain, side view.
PDB entry 4g2d coloured by chain, top view.
Source organism: Sulfolobus islandicus M.16.4
Primary publication:
Structural and enzymatic characterization of the lactonase SisLac from Sulfolobus islandicus.
Hiblot J, Gotthard G, Chabriere E, Elias M
PLoS One 7 e47028 (2012)
PMID: 23071703

Function and Biology Details

Reaction catalysed: 
An aryl dialkyl phosphate + H(2)O = dialkyl phosphate + an aryl alcohol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aryldialkylphosphatase Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 35.76 KDa
Source organism: Sulfolobus islandicus M.16.4
Expression system: Escherichia coli
UniProt:
  • Canonical: C4KKZ9 (Residues: 1-314; Coverage: 100%)
Gene name: M164_0332
Sequence domains: Phosphotriesterase family
Structure domains: Metal-dependent hydrolases

Ligands and Environments

2 bound ligands:
Ligand CO 1 x CO
Ligand FE2 1 x FE2
1 modified residue:
Ligand KCX 1 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P3221
Unit cell:
a: 47.81Å b: 47.81Å c: 239.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.266 0.265 0.292
Expression system: Escherichia coli

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Citations

13 review citations

Quorum quenching: role in nature and applied developments.
Grandclément et al. (2016)
12 more

2 mentions without citation

Structural and enzymatic characterization of the lactonase SisLac from Sulfolobus islandicus.
Hiblot et al. (2012)
1 more