› 4g3u

X-ray diffraction
2.69Å resolution

Mycobacterium smegmatis DprE1 - monoclinic crystal form

Released:
DOI: 10.2210/pdb4g3u/pdb
PDB entry 4g3u coloured by chain, front view.
PDB entry 4g3u coloured by chain, side view.
PDB entry 4g3u coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis
Primary publication:
Crystal structure of decaprenylphosphoryl-β- D-ribose 2'-epimerase from Mycobacterium smegmatis.
Li H, Jogl G
Proteins 81 538-43 (2013)
PMID: 23184707

Function and Biology Details

Reaction catalysed: 
Trans,octacis-decaprenylphospho-beta-D-ribofuranose + FAD = trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + FADH(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106479 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Decaprenylphosphoryl-beta-D-ribose oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 403 amino acids
Theoretical weight: 44.44 KDa
Source organism: Mycolicibacterium smegmatis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0R607 (Residues: 58-460; Coverage: 88%)
Gene names: LJ00_31545, MSMEG_6382, MSMEI_6214, dprE1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 105.152Å b: 65.808Å c: 108.504Å
α: 90° β: 109° γ: 90°
R-values:
R R work R free
0.222 0.22 0.254
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Why are membrane targets discovered by phenotypic screens and genome sequencing in Mycobacterium tuberculosis?
Goldman RC. (2013)
2 more

1 mention without citation

Crystal structure of decaprenylphosphoryl-β- D-ribose 2'-epimerase from Mycobacterium smegmatis.
Li et al. (2013)