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› 4g3v

X-ray diffraction
1.7Å resolution

Crystal structure of A. Aeolicus nlh2 gaf domain in an inactive state

Released:
DOI: 10.2210/pdb4g3v/pdb
Model geometry
Fit model/data
PDB entry 4g3v coloured by chain, front view.
PDB entry 4g3v coloured by chain, side view.
PDB entry 4g3v coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Structural mechanism of GAF-regulated σ(54) activators from Aquifex aeolicus.
Batchelor JD, Lee PS, Wang AC, Doucleff M, Wemmer DE
J Mol Biol 425 156-70 (2013)
PMID: 23123379

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130529 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sigma-54 factor interaction domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 172 amino acids
Theoretical weight: 19.41 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67661 (Residues: 1-172; Coverage: 35%)
Gene names: aq_1792, ntrC2
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P3221
Unit cell:
a: 65.45Å b: 65.45Å c: 128.59Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.165 0.163 0.206
Expression system: Escherichia coli

Quick links

Citations

2 review citations

CbbR, the Master Regulator for Microbial Carbon Dioxide Fixation.
Dangel et al. (2015)
1 more

1 mention without citation

Structural mechanism of GAF-regulated σ(54) activators from Aquifex aeolicus.
Batchelor et al. (2013)