› 4g4g

X-ray diffraction
1.55Å resolution

Crystal structure of recombinant glucuronoyl esterase from Sporotrichum thermophile determined at 1.55 A resolution

Released:
DOI: 10.2210/pdb4g4g/pdb
PDB entry 4g4g coloured by chain, front view.
PDB entry 4g4g coloured by chain, side view.
PDB entry 4g4g coloured by chain, top view.
Source organism: Thermothelomyces thermophilus ATCC 42464
Primary publication:
The structure of a novel glucuronoyl esterase from Myceliophthora thermophila gives new insights into its role as a potential biocatalyst.
Charavgi MD, Dimarogona M, Topakas E, Christakopoulos P, Chrysina ED
Acta Crystallogr D Biol Crystallogr 69 63-73 (2013)
PMID: 23275164

Function and Biology Details

Reaction catalysed: 
A 4-O-methyl-D-glucopyranuronate ester + H(2)O = 4-O-methyl-D-glucuronic acid + an alcohol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124293 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-O-methyl-glucuronoyl methylesterase Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 46.06 KDa
Source organism: Thermothelomyces thermophilus ATCC 42464
Expression system: Komagataella pastoris
UniProt:
  • Canonical: G2QJR6 (Residues: 1-397; Coverage: 100%)
Gene names: MYCTH_55568, ge2
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand EDO 14 x EDO
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P212121
Unit cell:
a: 45.973Å b: 58.735Å c: 136.157Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.254
Expression system: Komagataella pastoris

Quick links

Citations

5 review citations

Genomic insights into the fungal lignocellulolytic system of Myceliophthora thermophila.
Karnaouri et al. (2014)
4 more

8 mentions without citation

Carboxylic ester hydrolases: Classification and database derived from their primary, secondary, and tertiary structures.
Chen et al. (2016)
7 more