› 4g51

X-ray diffraction
2.5Å resolution

Crystallographic analysis of the interaction of nitric oxide with hemoglobin from Trematomus bernacchii in the T quaternary structure (fully ligated state).

Released:
DOI: 10.2210/pdb4g51/pdb
PDB entry 4g51 coloured by chain, front view.
PDB entry 4g51 coloured by chain, side view.
PDB entry 4g51 coloured by chain, top view.
Source organism: Trematomus bernacchii
Primary publication:
Selective X-ray-induced NO photodissociation in haemoglobin crystals: evidence from a Raman-assisted crystallographic study.
Merlino A, Fuchs MR, Pica A, Balsamo A, Dworkowski FS, Pompidor G, Mazzarella L, Vergara A
Acta Crystallogr D Biol Crystallogr 69 137-40 (2013)
PMID: 23275172

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160338 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hemoglobin subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 143 amino acids
Theoretical weight: 15.68 KDa
Source organism: Trematomus bernacchii
UniProt:
  • Canonical: P80043 (Residues: 1-142; Coverage: 100%)
Gene name: hba
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 146 amino acids
Theoretical weight: 16.15 KDa
Source organism: Trematomus bernacchii
UniProt:
  • Canonical: P80044 (Residues: 2-147; Coverage: 99%)
Gene name: hbb
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand NO 4 x NO
Ligand HEM 4 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 62.002Å b: 94.672Å c: 61.956Å
α: 90° β: 89.16° γ: 90°
R-values:
R R work R free
0.181 0.181 0.249

Quick links

Citations

1 review citation

From protein structure to function via single crystal optical spectroscopy.
Ronda et al. (2015)
4 other articles