› 4g5d

X-ray diffraction
1.8Å resolution

X-ray crystal structure of Prostaglandin f synthase from Leishmania major Friedlin bound to NADPH

Released:
DOI: 10.2210/pdb4g5d/pdb
PDB entry 4g5d coloured by chain, front view.
PDB entry 4g5d coloured by chain, side view.
PDB entry 4g5d coloured by chain, top view.
Source organism: Leishmania major strain Friedlin
Primary publication:
Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP.
Moen SO, Fairman JW, Barnes SR, Sullivan A, Nakazawa-Hewitt S, Van Voorhis WC, Staker BL, Lorimer DD, Myler PJ, Edwards TE
Acta Crystallogr F Struct Biol Commun 71 609-14 (2015)
PMID: 25945716

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
9,11-endoperoxide prostaglandin H2 reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 288 amino acids
Theoretical weight: 32.19 KDa
Source organism: Leishmania major strain Friedlin
Expression system: Escherichia coli
UniProt:
  • Canonical: P22045 (Residues: 1-284; Coverage: 100%)
Gene name: P100/11E
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments

1 bound ligand:
Ligand NDP 2 x NDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 94.66Å b: 34.59Å c: 106.95Å
α: 90° β: 102.98° γ: 90°
R-values:
R R work R free
0.174 0.172 0.218
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Polyunsaturated fatty acids alter the formation of lipid droplets and eicosanoid production in Leishmania promastigotes.
Andrade et al. (2023)
1 more

4 mentions without citation

Leishmania braziliensis prostaglandin F2α synthase impacts host infection.
Alves-Ferreira et al. (2020)
3 more