› 4g6u

X-ray diffraction
2.35Å resolution

CdiA-CT/CdiI toxin and immunity complex from Escherichia coli

Released:
DOI: 10.2210/pdb4g6u/pdb
PDB entry 4g6u coloured by chain, front view.
PDB entry 4g6u coloured by chain, side view.
PDB entry 4g6u coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Structural basis of toxicity and immunity in contact-dependent growth inhibition (CDI) systems.
Morse RP, Nikolakakis KC, Willett JL, Gerrick E, Low DA, Hayes CS, Goulding CW
Proc Natl Acad Sci U S A 109 21480-5 (2012)
PMID: 23236156

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123415 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
EC869 CdiA-CT Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 32.7 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: F2WK69 (Residues: 88-377; Coverage: 77%)
Sequence domains:
Structure domains:
DUF1436 family protein Chain: B
Molecule details ›
Chain: B
Length: 177 amino acids
Theoretical weight: 20.43 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: F2WK70 (Residues: 1-167; Coverage: 100%)
Sequence domains: CDI immunity protein
Structure domains: NMB0488-like

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand CL 1 x CL
Ligand ACT 3 x ACT
Ligand YT3 3 x YT3
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2221
Unit cell:
a: 81.699Å b: 103.631Å c: 125.173Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.229
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Ribosomally encoded antibacterial proteins and peptides from Pseudomonas.
Ghequire et al. (2014)
9 more

9 mentions without citation

Bacterial contact-dependent growth inhibition.
Ruhe et al. (2013)
8 more