› 4g6v

X-ray diffraction
2.64Å resolution

CdiA-CT/CdiI toxin and immunity complex from Burkholderia pseudomallei

Released:
DOI: 10.2210/pdb4g6v/pdb
PDB entry 4g6v coloured by chain, front view.
PDB entry 4g6v coloured by chain, side view.
PDB entry 4g6v coloured by chain, top view.
Source organism: Burkholderia pseudomallei 1026a
Primary publication:
Structural basis of toxicity and immunity in contact-dependent growth inhibition (CDI) systems.
Morse RP, Nikolakakis KC, Willett JL, Gerrick E, Low DA, Hayes CS, Goulding CW
Proc Natl Acad Sci U S A 109 21480-5 (2012)
PMID: 23236156

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124966 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Adhesin/hemolysin Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 176 amino acids
Theoretical weight: 18.23 KDa
Source organism: Burkholderia pseudomallei 1026a
Expression system: Escherichia coli
Structure domains: Trna Endonuclease; Chain: A, domain 1
CdiI Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 111 amino acids
Theoretical weight: 12.47 KDa
Source organism: Burkholderia pseudomallei 1026a
Expression system: Escherichia coli
UniProt:
  • Canonical: H9T8H3 (Residues: 1-101; Coverage: 100%)
Gene name: cdiI
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand BR 8 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: F222
Unit cell:
a: 151.963Å b: 173.654Å c: 174.822Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.245
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Ribosomally encoded antibacterial proteins and peptides from Pseudomonas.
Ghequire et al. (2014)
10 more

8 mentions without citation

Bacterial contact-dependent growth inhibition.
Ruhe et al. (2013)
7 more