› 4g7a

X-ray diffraction
1.8Å resolution

The crystal structure of an alpha Carbonic Anhydrase from the extremophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1

Released:
DOI: 10.2210/pdb4g7a/pdb
PDB entry 4g7a coloured by chain, front view.
PDB entry 4g7a coloured by chain, side view.
PDB entry 4g7a coloured by chain, top view.
Source organism: Sulfurihydrogenibium sp. YO3AOP1
Primary publication:
X-ray structure of the first `extremo-α-carbonic anhydrase', a dimeric enzyme from the thermophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1.
Di Fiore A, Capasso C, De Luca V, Monti SM, Carginale V, Supuran CT, Scozzafava A, Pedone C, Rossi M, De Simone G
Acta Crystallogr D Biol Crystallogr 69 1150-9 (2013)
PMID: 23695259

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241369 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonate dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 28.75 KDa
Source organism: Sulfurihydrogenibium sp. YO3AOP1
Expression system: Escherichia coli
Structure domains: Alpha carbonic anhydrase

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand AZM 2 x AZM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 48.5Å b: 68.36Å c: 74.69Å
α: 90° β: 106.03° γ: 90°
R-values:
R R work R free
0.168 0.168 0.203
Expression system: Escherichia coli

Quick links

Citations

14 review citations

An overview of the alpha-, beta- and gamma-carbonic anhydrases from Bacteria: can bacterial carbonic anhydrases shed new light on evolution of bacteria?
Capasso et al. (2015)
13 more

11 mentions without citation

Thermostable Carbonic Anhydrases in Biotechnological Applications.
Di Fiore et al. (2015)
10 more