› 4g7y

X-ray diffraction
2.8Å resolution

Crystal structure of voltage sensing domain of Ci-VSP with fragment antibody (R217E, 2.8 A)

Released:
DOI: 10.2210/pdb4g7y/pdb
PDB entry 4g7y coloured by chain, front view.
PDB entry 4g7y coloured by chain, side view.
PDB entry 4g7y coloured by chain, top view.
Source organisms:
Primary publication:
Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain.
Li Q, Wanderling S, Paduch M, Medovoy D, Singharoy A, McGreevy R, Villalba-Galea CA, Hulse RE, Roux B, Schulten K, Kossiakoff A, Perozo E
Nat Struct Mol Biol 21 244-52 (2014)
PMID: 24487958

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212270 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fragment antibody heavy chain Chain: H
Molecule details ›
Chain: H
Length: 219 amino acids
Theoretical weight: 23.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fragment antibody light chain Chain: L
Molecule details ›
Chain: L
Length: 211 amino acids
Theoretical weight: 23.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Voltage-sensor containing phosphatase Chain: S
Molecule details ›
Chain: S
Length: 185 amino acids
Theoretical weight: 21.17 KDa
Source organism: Ciona intestinalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4W8A1 (Residues: 89-260; Coverage: 30%)
Gene name: Ci-VSP
Sequence domains: Ion transport protein

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand MG 1 x MG
Ligand LDA 1 x LDA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B, APS BEAMLINE 24-ID-E
Spacegroup: P6522
Unit cell:
a: 121.01Å b: 121.01Å c: 229.57Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.206 0.261
Expression system: Escherichia coli

Quick links

Citations

45 review citations

The hitchhiker's guide to the voltage-gated sodium channel galaxy.
Ahern et al. (2016)
44 more

2 mentions without citation

Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain.
Li et al. (2014)
1 more