› 4g84

X-ray diffraction
2.4Å resolution

Crystal structure of human HisRS

Released:
DOI: 10.2210/pdb4g84/pdb
PDB entry 4g84 coloured by chain, front view.
PDB entry 4g84 coloured by chain, side view.
PDB entry 4g84 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Internally deleted human tRNA synthetase suggests evolutionary pressure for repurposing.
Xu Z, Wei Z, Zhou JJ, Ye F, Lo WS, Wang F, Lau CF, Wu J, Nangle LA, Chiang KP, Yang XL, Zhang M, Schimmel P
Structure 20 1470-7 (2012)
PMID: 22958643

Function and Biology Details

Reaction catalysed: 
ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146096 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histidine--tRNA ligase, cytoplasmic Chains: A, B
Molecule details ›
Chains: A, B
Length: 464 amino acids
Theoretical weight: 52.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12081 (Residues: 54-506; Coverage: 89%)
Gene names: HARS, HARS1, HRS
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand IMD 3 x IMD
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 93.519Å b: 93.519Å c: 254.533Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.25
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Essential nontranslational functions of tRNA synthetases.
Guo et al. (2013)
5 more

9 mentions without citation

Structural disorder in expanding the functionome of aminoacyl-tRNA synthetases.
Yang XL. (2013)
8 more