› 4g8l

X-ray diffraction
2.8Å resolution

Active state of intact sensor domain of human RNase L with 2-5A bound

Released:
DOI: 10.2210/pdb4g8l/pdb
PDB entry 4g8l coloured by chain, front view.
PDB entry 4g8l coloured by chain, side view.
PDB entry 4g8l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Innate immune messenger 2-5A tethers human RNase L into active high-order complexes.
Han Y, Whitney G, Donovan J, Korennykh A
Cell Rep 2 902-13 (2012)
PMID: 23084743

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-5A-dependent ribonuclease Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 337 amino acids
Theoretical weight: 37.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05823 (Residues: 1-337; Coverage: 46%)
Gene names: RNASEL, RNS4
Sequence domains: Ankyrin repeat
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

1 bound ligand:
Ligand 25A 4 x 25A
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 62.98Å b: 164.11Å c: 86.22Å
α: 90° β: 100.2° γ: 90°
R-values:
R R work R free
0.23 0.228 0.261
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Inhibition of the OAS/RNase L pathway by viruses.
Drappier et al. (2015)
6 more

1 mention without citation

Dimeric structure of pseudokinase RNase L bound to 2-5A reveals a basis for interferon-induced antiviral activity.
Huang et al. (2014)