› 4gaj

X-ray diffraction
2.51Å resolution

Structure of the broadly neutralizing antibody AP33 in complex with its HCV epitope (E2 residues 411-424)

Released:
DOI: 10.2210/pdb4gaj/pdb
PDB entry 4gaj coloured by chain, front view.
PDB entry 4gaj coloured by chain, side view.
PDB entry 4gaj coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Toward a hepatitis C virus vaccine: the structural basis of hepatitis C virus neutralization by AP33, a broadly neutralizing antibody.
Potter JA, Owsianka AM, Jeffery N, Matthews DJ, Keck ZY, Lau P, Foung SK, Taylor GL, Patel AH
J Virol 86 12923-32 (2012)
PMID: 22993159

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212760 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
NEUTRALIZING ANTIBODY AP33 HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 218 amino acids
Theoretical weight: 23.68 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
NEUTRALIZING ANTIBODY AP33 LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 23.92 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Genome polyprotein Chain: P
Molecule details ›
Chain: P
Length: 14 amino acids
Theoretical weight: 1.67 KDa
UniProt:
  • Canonical: Q9IY15 (Residues: 94-107; Coverage: 8%)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 171.91Å b: 40.67Å c: 73.77Å
α: 90° β: 112.11° γ: 90°
R-values:
R R work R free
0.244 0.24 0.32

Quick links

Citations

27 review citations

The past, present and future of neutralizing antibodies for hepatitis C virus.
Ball et al. (2014)
26 more

11 mentions without citation

HCV E2 core structures and mAbs: something is still missing.
Castelli et al. (2014)
10 more