› 4gbg

X-ray diffraction
2.9Å resolution

Crystal structure of Ethyl acetoacetate treated lipase from Thermomyces lanuginosa at 2.9 A resolution

Released:
DOI: 10.2210/pdb4gbg/pdb
PDB entry 4gbg coloured by chain, front view.
PDB entry 4gbg coloured by chain, side view.
PDB entry 4gbg coloured by chain, top view.
Source organism: Thermomyces lanuginosus
Entry authors: Yamini S, Mukherjee J, Gupta MN, Sinha M, Kaur P, Sharma S, Singh TP

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129891 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 29.34 KDa
Source organism: Thermomyces lanuginosus
UniProt:
  • Canonical: O59952 (Residues: 23-291; Coverage: 98%)
Gene name: LIP
Sequence domains:
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand EAC 1 x EAC
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P61
Unit cell:
a: 140.489Å b: 140.489Å c: 80.69Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.226 0.247

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