› 4gc0

X-ray diffraction
2.6Å resolution

The structure of the MFS (major facilitator superfamily) proton:xylose symporter XylE bound to 6-bromo-6-deoxy-D-glucose

Released:
DOI: 10.2210/pdb4gc0/pdb
PDB entry 4gc0 coloured by chain, front view.
PDB entry 4gc0 coloured by chain, side view.
PDB entry 4gc0 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of a bacterial homologue of glucose transporters GLUT1-4.
Sun L, Zeng X, Yan C, Sun X, Gong X, Rao Y, Yan N
Nature 490 361-6 (2012)
PMID: 23075985

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142832 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-xylose-proton symporter Chain: A
Molecule details ›
Chain: A
Length: 491 amino acids
Theoretical weight: 53.64 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGF4 (Residues: 1-491; Coverage: 100%)
Gene names: JW3991, b4031, xylE
Sequence domains: Sugar (and other) transporter
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

2 bound ligands:
Ligand 6BG 1 x 6BG
Ligand BNG 4 x BNG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 95.171Å b: 95.171Å c: 171.668Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.227 0.246
Expression system: Escherichia coli

Quick links

Citations

47 review citations

Blood-Brain Barrier: From Physiology to Disease and Back.
Sweeney et al. (2019)
46 more

28 mentions without citation

GLUT, SGLT, and SWEET: Structural and mechanistic investigations of the glucose transporters.
Deng et al. (2016)
27 more