› 4gcl

X-ray diffraction
2.65Å resolution

structure of no-dna factor

Released:
DOI: 10.2210/pdb4gcl/pdb
PDB entry 4gcl coloured by chain, front view.
PDB entry 4gcl coloured by chain, side view.
PDB entry 4gcl coloured by chain, top view.
Source organism: Escherichia coli UTI89
Primary publication:
SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid.
Tonthat NK, Milam SL, Chinnam N, Whitfill T, Margolin W, Schumacher MA
Proc Natl Acad Sci U S A 110 10586-91 (2013)
PMID: 23754405

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117539 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Nucleoid occlusion factor SlmA Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 212 amino acids
Theoretical weight: 24.32 KDa
Source organism: Escherichia coli UTI89
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1R4V1 (Residues: 1-212; Coverage: 100%)
Gene names: UTI89_C4185, slmA, ttk
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2
DNA (5'-D(*AP*GP*TP*GP*AP*GP*TP*AP*CP*TP*CP*AP*CP*T)-3') Chains: R, T, W, Z
Molecule details ›
Chains: R, T, W, Z
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
Source organism: Escherichia coli UTI89
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MES 4 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 70.29Å b: 160.52Å c: 201.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.276
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

17 review citations

Subcellular Organization: A Critical Feature of Bacterial Cell Replication.
Surovtsev et al. (2018)
16 more

3 mentions without citation

SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid.
Tonthat et al. (2013)
2 more