› 4gcq

X-ray diffraction
2.2Å resolution

Crystal Structure of E. coli OmpF porin in complex with Carbenicillin

Released:
DOI: 10.2210/pdb4gcq/pdb
PDB entry 4gcq coloured by chain, front view.
PDB entry 4gcq coloured by chain, side view.
PDB entry 4gcq coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The binding of antibiotics in OmpF porin.
Ziervogel BK, Roux B
Structure 21 76-87 (2013)
PMID: 23201272

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136399 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane porin F Chains: A, B
Molecule details ›
Chains: A, B
Length: 341 amino acids
Theoretical weight: 37.17 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P02931 (Residues: 23-362; Coverage: 100%)
Gene names: JW0912, b0929, cmlB, coa, cry, ompF, tolF
Sequence domains: Gram-negative porin
Structure domains: Porin

Ligands and Environments

1 bound ligand:
Ligand 0JM 2 x 0JM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P3
Unit cell:
a: 116.71Å b: 116.71Å c: 53.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.219 0.275
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Porins and small-molecule translocation across the outer membrane of Gram-negative bacteria.
Vergalli et al. (2020)
9 more

6 mentions without citation

Recent Advances in Exploration and Biotechnological Production of Bioactive Compounds in Three Cyanobacterial Genera: Nostoc, Lyngbya, and Microcystis.
Thuan et al. (2019)
5 more