› 4gcy

X-ray diffraction
1.5Å resolution

Structure of Mycobacterium tuberculosis dUTPase H21W mutant

Released:
DOI: 10.2210/pdb4gcy/pdb
PDB entry 4gcy coloured by chain, front view.
PDB entry 4gcy coloured by chain, side view.
PDB entry 4gcy coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Entry authors: Toth J, Vertessy BG, Leveles I, Bendes A

Function and Biology Details

Reaction catalysed: 
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161882 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyuridine 5'-triphosphate nucleotidohydrolase Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 18.04 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WNS5 (Residues: 1-154; Coverage: 100%)
Gene names: MTCY05A6.18c, Rv2697c, dut
Sequence domains: dUTPase
Structure domains: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase; Chain A

Ligands and Environments

4 bound ligands:
Ligand DUP 1 x DUP
Ligand TRS 1 x TRS
Ligand MG 1 x MG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P63
Unit cell:
a: 54.94Å b: 54.94Å c: 83.86Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.125 0.123 0.158
Expression system: Escherichia coli BL21(DE3)

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