› 4ge1

X-ray diffraction
2.15Å resolution

Structure of the tryptamine complex of the amine binding protein of Rhodnius prolixus

Released:
DOI: 10.2210/pdb4ge1/pdb
PDB entry 4ge1 coloured by chain, front view.
PDB entry 4ge1 coloured by chain, side view.
PDB entry 4ge1 coloured by chain, top view.
Source organism: Rhodnius prolixus
Primary publication:
Structure and ligand-binding properties of the biogenic amine-binding protein from the saliva of a blood-feeding insect vector of Trypanosoma cruzi.
Xu X, Chang BW, Mans BJ, Ribeiro JM, Andersen JF
Acta Crystallogr D Biol Crystallogr 69 105-13 (2013)
PMID: 23275168

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 197 amino acids
Theoretical weight: 22.24 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86PT9 (Residues: 22-217; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand TSS 4 x TSS
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 69.257Å b: 73.086Å c: 80.935Å
α: 90° β: 91.45° γ: 90°
R-values:
R R work R free
0.195 0.192 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Insights into the sand fly saliva: Blood-feeding and immune interactions between sand flies, hosts, and Leishmania.
Lestinova et al. (2017)
1 more

3 mentions without citation

Structure and ligand-binding properties of the biogenic amine-binding protein from the saliva of a blood-feeding insect vector of Trypanosoma cruzi.
Xu et al. (2013)
2 more