› 4geg

X-ray diffraction
2.49Å resolution

Crystal Structure of E.coli MenH Y85F Mutant

Released:
DOI: 10.2210/pdb4geg/pdb
PDB entry 4geg coloured by chain, front view.
PDB entry 4geg coloured by chain, side view.
PDB entry 4geg coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structures of E. coli native MenH and two active site mutants.
Johnston JM, Jiang M, Guo Z, Baker EN
PLoS One 8 e61325 (2013)
PMID: 23637813

Function and Biology Details

Reaction catalysed: 
5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153402 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 268 amino acids
Theoretical weight: 29.5 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37355 (Residues: 1-252; Coverage: 100%)
Gene names: JW2258, b2263, menH, yfbB
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

4 bound ligands:
Ligand SO4 13 x SO4
Ligand CL 7 x CL
Ligand EDO 1 x EDO
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: C2
Unit cell:
a: 250.499Å b: 41.589Å c: 93.081Å
α: 90° β: 95.89° γ: 90°
R-values:
R R work R free
0.18 0.177 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Biosynthesis and applications of prenylquinones.
Kawamukai M. (2018)
3 other articles

1 mention without citation

Crystal structures of E. coli native MenH and two active site mutants.
Johnston et al. (2013)