› 4gen

X-ray diffraction
2.2Å resolution

Crystal structure of Zucchini (monomer)

Released:
DOI: 10.2210/pdb4gen/pdb
PDB entry 4gen coloured by chain, front view.
PDB entry 4gen coloured by chain, side view.
PDB entry 4gen coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structure and function of Zucchini endoribonuclease in piRNA biogenesis.
Nishimasu H, Ishizu H, Saito K, Fukuhara S, Kamatani MK, Bonnefond L, Matsumoto N, Nishizawa T, Nakanaga K, Aoki J, Ishitani R, Siomi H, Siomi MC, Nureki O
Nature 491 284-7 (2012)
PMID: 23064230

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194272 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitochondrial cardiolipin hydrolase Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.35 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VKD7 (Residues: 89-250; Coverage: 64%)
Gene names: CG12314, zuc
Sequence domains: PLD-like domain
Structure domains: Endonuclease Chain A

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P21
Unit cell:
a: 35.277Å b: 52.396Å c: 38.791Å
α: 90° β: 104.94° γ: 90°
R-values:
R R work R free
0.225 0.223 0.27
Expression system: Escherichia coli

Quick links

Citations

84 review citations

Regulation of microRNA biogenesis.
Ha et al. (2014)
83 more

5 mentions without citation

Dimeric interactions and complex formation using direct coevolutionary couplings.
dos Santos et al. (2015)
4 more