› 4ger

X-ray diffraction
1.59Å resolution

Crystal structure of Gentlyase, the neutral metalloprotease of Paenibacillus polymyxa

Released:
DOI: 10.2210/pdb4ger/pdb
PDB entry 4ger coloured by chain, front view.
PDB entry 4ger coloured by chain, side view.
PDB entry 4ger coloured by chain, top view.
Source organism: Paenibacillus polymyxa
Primary publication:
Structure of gentlyase, the neutral metalloprotease of Paenibacillus polymyxa.
Ruf A, Stihle M, Benz J, Schmidt M, Sobek H
Acta Crystallogr D Biol Crystallogr 69 24-31 (2013)
PMID: 23275160

Function and Biology Details

Reaction catalysed: 
Similar, but not identical, to that of thermolysin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151358 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacillolysin Chains: A, B
Molecule details ›
Chains: A, B
Length: 304 amino acids
Theoretical weight: 32.41 KDa
Source organism: Paenibacillus polymyxa
Expression system: Bacillus amyloliquefaciens
UniProt:
  • Canonical: P29148 (Residues: 289-590; Coverage: 53%)
Gene name: npr
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 6 x CA
Ligand THR 2 x THR
Ligand LYS 2 x LYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 61.014Å b: 77.07Å c: 64.052Å
α: 90° β: 103.16° γ: 90°
R-values:
R R work R free
0.202 0.2 0.243
Expression system: Bacillus amyloliquefaciens

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Citations

1 review citation

Bacterial and Fungal Proteolytic Enzymes: Production, Catalysis and Potential Applications.
da Silva RR. (2017)
5 other articles

2 mentions without citation

Upgraded AMBER Force Field for Zinc-Binding Residues and Ligands for Predicting Structural Properties and Binding Affinities in Zinc-Proteins.
Macchiagodena et al. (2020)
1 more