› 4gey

X-ray diffraction
2.7Å resolution

High pH structure of Pseudomonas putida OprB

Released:
DOI: 10.2210/pdb4gey/pdb
PDB entry 4gey coloured by chain, front view.
PDB entry 4gey coloured by chain, side view.
PDB entry 4gey coloured by chain, top view.
Source organism: Pseudomonas putida BIRD-1
Primary publication:
Structural basis for outer membrane sugar uptake in pseudomonads.
van den Berg B
J Biol Chem 287 41044-52 (2012)
PMID: 23066028

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107762 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Porin, OprB family Chain: A
Molecule details ›
Chain: A
Length: 436 amino acids
Theoretical weight: 49.05 KDa
Source organism: Pseudomonas putida BIRD-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5VZA8 (Residues: 27-447; Coverage: 100%)
Gene name: Pput_1057
Sequence domains: Carbohydrate-selective porin, OprB family

Ligands and Environments

4 bound ligands:
Ligand BGC 1 x BGC
Ligand C8E 8 x C8E
Ligand DMU 4 x DMU
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P3121
Unit cell:
a: 88.343Å b: 88.343Å c: 177.706Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.188 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Permeability barriers of Gram-negative pathogens.
Zgurskaya et al. (2020)
3 more

3 mentions without citation

Structural basis for outer membrane sugar uptake in pseudomonads.
van den Berg B. (2012)
2 more