› 4gf3

X-ray diffraction
1.9Å resolution

Structure of a SycH-YopH Chaperone-Effector Complex

Released:
DOI: 10.2210/pdb4gf3/pdb
PDB entry 4gf3 coloured by chain, front view.
PDB entry 4gf3 coloured by chain, side view.
PDB entry 4gf3 coloured by chain, top view.
Source organisms:
Primary publication:
Context-dependent protein folding of a virulence peptide in the bacterial and host environments: structure of an SycH-YopH chaperone-effector complex.
Vujanac M, Stebbins CE
Acta Crystallogr D Biol Crystallogr 69 546-54 (2013)
PMID: 23519663

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147290 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Putative yopH targeting protein SycH Chain: A
Molecule details ›
Chain: A
Length: 141 amino acids
Theoretical weight: 15.78 KDa
Source organism: Yersinia pestis
Expression system: Not provided
UniProt:
  • Canonical: Q7BTX0 (Residues: 1-141; Coverage: 100%)
Gene names: Y0082, YPCD1.95c, sycH
Sequence domains: Tir chaperone protein (CesT) family
Structure domains: Yope Regulator; Chain: A,
Tyrosine-protein phosphatase YopH Chain: B
Molecule details ›
Chain: B
Length: 43 amino acids
Theoretical weight: 4.5 KDa
Source organism: Yersinia enterocolitica
Expression system: Not provided
UniProt:
  • Canonical: P15273 (Residues: 21-63; Coverage: 9%)
Gene names: yop51, yopH
Sequence domains: YopH, N-terminal

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P41212
Unit cell:
a: 70.525Å b: 70.525Å c: 71.517Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.245
Expression system: Not provided

Quick links

Citations

3 review citations

Assembly and structure of the T3SS.
Burkinshaw et al. (2014)
2 more

4 mentions without citation

Structure of the HopA1(21-102)-ShcA chaperone-effector complex of Pseudomonas syringae reveals conservation of a virulence factor binding motif from animal to plant pathogens.
Janjusevic et al. (2013)
3 more