› 4gf4

X-ray diffraction
3.1Å resolution

Low pH structure of Pseudomonas putida OprB

Released:
DOI: 10.2210/pdb4gf4/pdb
PDB entry 4gf4 coloured by chain, front view.
PDB entry 4gf4 coloured by chain, side view.
PDB entry 4gf4 coloured by chain, top view.
Source organism: Pseudomonas putida BIRD-1
Primary publication:
Structural basis for outer membrane sugar uptake in pseudomonads.
van den Berg B
J Biol Chem 287 41044-52 (2012)
PMID: 23066028

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107762 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Porin, OprB family Chain: A
Molecule details ›
Chain: A
Length: 436 amino acids
Theoretical weight: 49.18 KDa
Source organism: Pseudomonas putida BIRD-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5VZA8 (Residues: 27-447; Coverage: 100%)
Gene name: Pput_1057
Sequence domains: Carbohydrate-selective porin, OprB family
Structure domains: Carbohydrate-selective porin OprB

Ligands and Environments

1 bound ligand:
Ligand C8E 4 x C8E
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: I222
Unit cell:
a: 79.037Å b: 122.592Å c: 135.581Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.214 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Permeability barriers of Gram-negative pathogens.
Zgurskaya et al. (2020)
3 more

1 mention without citation

Structural basis for outer membrane sugar uptake in pseudomonads.
van den Berg B. (2012)