› 4gfl

X-ray diffraction
2.3Å resolution

NO mechanism, slma

Released:
DOI: 10.2210/pdb4gfl/pdb
PDB entry 4gfl coloured by chain, front view.
PDB entry 4gfl coloured by chain, side view.
PDB entry 4gfl coloured by chain, top view.
Source organism: Klebsiella pneumoniae 342
Primary publication:
SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid.
Tonthat NK, Milam SL, Chinnam N, Whitfill T, Margolin W, Schumacher MA
Proc Natl Acad Sci U S A 110 10586-91 (2013)
PMID: 23754405

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoid occlusion factor SlmA Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 24.38 KDa
Source organism: Klebsiella pneumoniae 342
Expression system: Escherichia coli
UniProt:
  • Canonical: B5XTG2 (Residues: 1-198; Coverage: 100%)
Gene names: KPK_0113, slmA
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 71.92Å b: 40.86Å c: 76.43Å
α: 90° β: 111.8° γ: 90°
R-values:
R R work R free
0.238 0.238 0.278
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Subcellular Organization: A Critical Feature of Bacterial Cell Replication.
Surovtsev et al. (2018)
16 more

2 mentions without citation

SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid.
Tonthat et al. (2013)
1 more