› 4gg4

X-ray diffraction
2.5Å resolution

Crystal structure of the TAL effector dHax3 bound to specific DNA-RNA hybrid

Released:
DOI: 10.2210/pdb4gg4/pdb
PDB entry 4gg4 coloured by chain, front view.
PDB entry 4gg4 coloured by chain, side view.
PDB entry 4gg4 coloured by chain, top view.
Source organism: Xanthomonas campestris pv. armoraciae
Primary publication:
Specific DNA-RNA hybrid recognition by TAL effectors.
Yin P, Deng D, Yan C, Pan X, Xi JJ, Yan N, Shi Y
Cell Rep 2 707-13 (2012)
PMID: 23022487

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117544 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (3 distinct):
Avirulence protein AvrBs3 Chain: A
Molecule details ›
Chain: A
Length: 499 amino acids
Theoretical weight: 51.78 KDa
Source organism: Xanthomonas campestris pv. armoraciae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3ZD72 (Residues: 231-720; Coverage: 51%)
Gene name: hax3
Sequence domains: TAL effector repeat
DNA (5'-D(*TP*GP*TP*CP*CP*CP*TP*TP*TP*AP*TP*CP*TP*CP*TP*CP*T)-3') Chain: G
Molecule details ›
Chain: G
Length: 17 nucleotides
Theoretical weight: 5.07 KDa
Source organism: Xanthomonas campestris pv. armoraciae
Expression system: Not provided
RNA (5'-R(*AP*GP*AP*GP*AP*GP*AP*UP*AP*AP*AP*GP*GP*GP*AP*CP*A)-3') Chain: H
Molecule details ›
Chain: H
Length: 17 nucleotides
Theoretical weight: 5.6 KDa
Source organism: Xanthomonas campestris pv. armoraciae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P61
Unit cell:
a: 99.735Å b: 99.735Å c: 134.494Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.193 0.243
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

TAL effectors: highly adaptable phytobacterial virulence factors and readily engineered DNA-targeting proteins.
Doyle et al. (2013)
8 more

3 mentions without citation

Cytosolic RNA:DNA hybrids activate the cGAS-STING axis.
Mankan et al. (2014)
2 more