› 4ggj

X-ray diffraction
1.75Å resolution

Crystal structure of Zucchini from mouse (mZuc / PLD6 / MitoPLD)

Released:
DOI: 10.2210/pdb4ggj/pdb
PDB entry 4ggj coloured by chain, front view.
PDB entry 4ggj coloured by chain, side view.
PDB entry 4ggj coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The structural biochemistry of Zucchini implicates it as a nuclease in piRNA biogenesis.
Ipsaro JJ, Haase AD, Knott SR, Joshua-Tor L, Hannon GJ
Nature 491 279-83 (2012)
PMID: 23064227

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178284 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitochondrial cardiolipin hydrolase Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 22.34 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q5SWZ9 (Residues: 31-221; Coverage: 86%)
Gene name: Pld6
Sequence domains: PLD-like domain
Structure domains: Endonuclease Chain A

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P43212
Unit cell:
a: 38.741Å b: 38.741Å c: 214.529Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.197
Expression system: Spodoptera frugiperda

Quick links

Citations

73 review citations

Regulation of microRNA biogenesis.
Ha et al. (2014)
72 more

5 mentions without citation

From guide to target: molecular insights into eukaryotic RNA-interference machinery.
Ipsaro et al. (2015)
4 more