› 4ghl

X-ray diffraction
2.02Å resolution

Structural Basis for Marburg virus VP35 mediate immune evasion mechanisms

Released:
DOI: 10.2210/pdb4ghl/pdb
PDB entry 4ghl coloured by chain, front view.
PDB entry 4ghl coloured by chain, side view.
PDB entry 4ghl coloured by chain, top view.
Source organism: Lake Victoria marburgvirus - Popp
Primary publication:
Structural basis for Marburg virus VP35-mediated immune evasion mechanisms.
Ramanan P, Edwards MR, Shabman RS, Leung DW, Endlich-Frazier AC, Borek DM, Otwinowski Z, Liu G, Huh J, Basler CF, Amarasinghe GK
Proc Natl Acad Sci U S A 109 20661-6 (2012)
PMID: 23185024

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-219589 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Polymerase cofactor VP35 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 133 amino acids
Theoretical weight: 14.64 KDa
Source organism: Lake Victoria marburgvirus - Popp
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03039 (Residues: 200-329; Coverage: 40%)
Gene name: VP35
Sequence domains: Filoviridae VP35
Structure domains:
short palindromic RNA AGACAGCAUAUGCUGUCU Chains: E, F
Molecule details ›
Chains: E, F
Length: 18 nucleotides
Theoretical weight: 5.73 KDa
Source organism: Lake Victoria marburgvirus - Popp
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand MG 11 x MG
Ligand EDO 4 x EDO
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 83.771Å b: 93.384Å c: 102.071Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

32 review citations

Viral evasion of intracellular DNA and RNA sensing.
Chan et al. (2016)
31 more

9 mentions without citation

Filovirus pathogenesis and immune evasion: insights from Ebola virus and Marburg virus.
Messaoudi et al. (2015)
8 more