› 4gjg

X-ray diffraction
2Å resolution

Crystal structure of the amino-terminal domain of human cardiac troponin C mutant D2N/V28I/L29Q/G30D (NIQD) in complex with cadmium.

Released:
DOI: 10.2210/pdb4gjg/pdb
PDB entry 4gjg coloured by chain, front view.
PDB entry 4gjg coloured by chain, side view.
PDB entry 4gjg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of cardiac troponin C regulatory domain with bound Cd2+ reveals a closed conformation and unique ion coordination.
Zhang XL, Tibbits GF, Paetzel M
Acta Crystallogr D Biol Crystallogr 69 722-34 (2013)
PMID: 23633581

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-159088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Troponin C, slow skeletal and cardiac muscles Chain: A
Molecule details ›
Chain: A
Length: 89 amino acids
Theoretical weight: 10.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63316 (Residues: 1-89; Coverage: 55%)
Gene names: TNNC, TNNC1
Sequence domains: EF-hand domain pair

Ligands and Environments

4 bound ligands:
Ligand CD 7 x CD
Ligand CA 3 x CA
Ligand ACT 1 x ACT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P6122
Unit cell:
a: 49.583Å b: 49.583Å c: 115.141Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.146 0.144 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Structure and function of cardiac troponin C (TNNC1): Implications for heart failure, cardiomyopathies, and troponin modulating drugs.
Li et al. (2015)
1 more