› 4gjr

X-ray diffraction
1.85Å resolution

Crystal structure of the TAL effector dHax3 bound to methylated dsDNA

Released:
DOI: 10.2210/pdb4gjr/pdb
PDB entry 4gjr coloured by chain, front view.
PDB entry 4gjr coloured by chain, side view.
PDB entry 4gjr coloured by chain, top view.
Source organism: Xanthomonas campestris pv. armoraciae
Entry authors: Yan N, Deng D, Yan CY, Yin P, Pan XJ, Shi YG

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117546 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Avirulence protein AvrBs3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 499 amino acids
Theoretical weight: 51.75 KDa
Source organism: Xanthomonas campestris pv. armoraciae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3ZD72 (Residues: 231-720; Coverage: 51%)
Gene name: hax3
Sequence domains: TAL effector repeat
DNA (5'-D(*TP*GP*TP*CP*CP*CP*TP*(5CM)P*TP*AP*(5CM)P*CP*TP*CP*(5CM)P*CP*T)-3') Chains: G, I
Molecule details ›
Chains: G, I
Length: 17 nucleotides
Theoretical weight: 5.07 KDa
Source organism: Xanthomonas campestris pv. armoraciae
Expression system: Not provided
DNA (5'-D(*AP*GP*GP*GP*AP*GP*GP*TP*AP*GP*AP*GP*GP*GP*AP*CP*A)-3') Chains: H, J
Molecule details ›
Chains: H, J
Length: 17 nucleotides
Theoretical weight: 5.39 KDa
Source organism: Xanthomonas campestris pv. armoraciae
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 5CM 6 x 5CM

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 81.839Å b: 87.625Å c: 88.455Å
α: 90° β: 102.85° γ: 90°
R-values:
R R work R free
0.2 0.198 0.23
Expression systems:
  • Escherichia coli
  • Not provided

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