› 4gkf

X-ray diffraction
2.1Å resolution

Crystal structure and characterization of Cmr5 protein from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb4gkf/pdb
PDB entry 4gkf coloured by chain, front view.
PDB entry 4gkf coloured by chain, side view.
PDB entry 4gkf coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Crystal structure of Cmr5 from Pyrococcus furiosus and its functional implications.
Park JH, Sun J, Park SY, Hwang HJ, Park MY, Shin M, Kim JS
FEBS Lett 587 562-8 (2013)
PMID: 23370277

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR system Cmr subunit Cmr5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 19.72 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U1T0 (Residues: 1-169; Coverage: 100%)
Gene names: PF1125, cmr5
Sequence domains: CRISPR-associated protein (Cas_Cmr5)
Structure domains: AF1862-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 53.944Å b: 53.944Å c: 235.987Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.276
Expression system: Escherichia coli

Quick links

Citations

2 review citations

DNA and RNA interference mechanisms by CRISPR-Cas surveillance complexes.
Plagens et al. (2015)
1 more

4 mentions without citation

CRISPR interference: a structural perspective.
Reeks et al. (2013)
3 more