› 4gkr

X-ray diffraction
2.69Å resolution

Structure of the C-terminal motor domain of Kar3 from Candida glabrata

Released:
DOI: 10.2210/pdb4gkr/pdb
PDB entry 4gkr coloured by chain, front view.
PDB entry 4gkr coloured by chain, side view.
PDB entry 4gkr coloured by chain, top view.
Source organism: [Candida] glabrata CBS 138
Primary publication:
Neck rotation and neck mimic docking in the noncatalytic Kar3-associated protein Vik1.
Duan D, Jia Z, Joshi M, Brunton J, Chan M, Drew D, Davis D, Allingham JS
J Biol Chem 287 40292-301 (2012)
PMID: 23043140

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin motor domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 371 amino acids
Theoretical weight: 41.9 KDa
Source organism: [Candida] glabrata CBS 138
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6FVW6 (Residues: 324-692; Coverage: 53%)
Gene names: CAGL0D04994g, KAR3
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ADP 2 x ADP
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 58.707Å b: 84.066Å c: 151.127Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.21 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Molecular mechanisms of kinesin-14 motors in spindle assembly and chromosome segregation.
She et al. (2017)
6 other articles

2 mentions without citation

Kinesin Motor Enzymology: Chemistry, Structure, and Physics of Nanoscale Molecular Machines.
Cochran JC. (2015)
1 more