› 4glk

X-ray diffraction
2.16Å resolution

Structure and activity of AbiQ, a lactococcal anti-phage endoribonuclease belonging to the type-III toxin-antitoxin system

Released:
DOI: 10.2210/pdb4glk/pdb
PDB entry 4glk coloured by chain, front view.
PDB entry 4glk coloured by chain, side view.
PDB entry 4glk coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
Structure and activity of AbiQ, a lactococcal endoribonuclease belonging to the type III toxin-antitoxin system.
Samson JE, Spinelli S, Cambillau C, Moineau S
Mol Microbiol 87 756-68 (2013)
PMID: 23279123

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195663 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoribonuclease AbiQ Chain: A
Molecule details ›
Chain: A
Length: 172 amino acids
Theoretical weight: 20.31 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q9ZJ19 (Residues: 13-183; Coverage: 93%)
Gene name: abiQ
Sequence domains: Toxin ToxN, type III toxin-antitoxin system
Structure domains: Thiol Ester Dehydrase; Chain A

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 50.14Å b: 54.51Å c: 64.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.223
Expression system: Escherichia coli K-12

Quick links

Citations

19 review citations

Revenge of the phages: defeating bacterial defences.
Samson et al. (2013)
18 more

3 mentions without citation

Structure, Evolution, and Functions of Bacterial Type III Toxin-Antitoxin Systems.
Goeders et al. (2016)
2 more