› 4glu

X-ray diffraction
1.9Å resolution

Crystal structure of the mirror image form of VEGF-A

Released:
DOI: 10.2210/pdb4glu/pdb
PDB entry 4glu coloured by chain, front view.
PDB entry 4glu coloured by chain, side view.
PDB entry 4glu coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Chemical synthesis and X-ray structure of a heterochiral {D-protein antagonist plus vascular endothelial growth factor} protein complex by racemic crystallography.
Mandal K, Uppalapati M, Ault-Riché D, Kenney J, Lowitz J, Sidhu SS, Kent SB
Proc Natl Acad Sci U S A 109 14779-84 (2012)
PMID: 22927390

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-164508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D- Vascular endothelial growth factor-A Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 102 amino acids
Theoretical weight: 11.94 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ACT 10 x ACT
Ligand TFA 7 x TFA
Ligand GOL 4 x GOL
17 modified residues:
Ligand DGN 42 x DGN
Ligand DSG 24 x DSG
Ligand DHI 36 x DHI
Ligand DGL 66 x DGL
Ligand DVA 36 x DVA
Ligand DLY 36 x DLY
Ligand DPN 24 x DPN
Ligand MED 30 x MED
Ligand DAS 30 x DAS
Ligand DTY 24 x DTY
Ligand DAR 24 x DAR
Ligand DSN 24 x DSN
Ligand DCY 48 x DCY
Ligand DPR 42 x DPR
Ligand DIL 48 x DIL
Ligand DTH 18 x DTH
Ligand DLE 24 x DLE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 231.395Å b: 43.986Å c: 73.514Å
α: 90° β: 100.3° γ: 90°
R-values:
R R work R free
0.174 0.172 0.209
Expression system: Not provided

Quick links

Citations

13 review citations

Expanding the chemical toolbox for the synthesis of large and uniquely modified proteins.
Bondalapati et al. (2016)
12 more

12 mentions without citation

Molecular imaging of the urokinase plasminogen activator receptor: opportunities beyond cancer.
Baart et al. (2020)
11 more