› 4gn3

X-ray diffraction
1.95Å resolution

OBody AM1L10 bound to hen egg-white lysozyme

Released:
DOI: 10.2210/pdb4gn3/pdb
Model geometry
Fit model/data
PDB entry 4gn3 coloured by chain, front view.
PDB entry 4gn3 coloured by chain, side view.
PDB entry 4gn3 coloured by chain, top view.
Source organisms:
Primary publication:
Tracking molecular recognition at the atomic level with a new protein scaffold based on the OB-fold.
Steemson JD, Baake M, Rakonjac J, Arcus VL, Liddament MT
OpenAccess logo PLoS One 9 e86050 (2014)
PMID: 24465865

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132854 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lysozyme C Chains: A, C, E, G, I, K, M, O, Q
Molecule details ›
Chains: A, C, E, G, I, K, M, O, Q
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme
OBody AM1L10 Chains: B, D, F, H, J, L, N, P, R
Molecule details ›
Chains: B, D, F, H, J, L, N, P, R
Length: 113 amino acids
Theoretical weight: 12.31 KDa
Source organism: Pyrobaculum aerophilum
Expression system: Escherichia coli DH5[alpha]
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

2 bound ligands:
Ligand GOL 18 x GOL
Ligand EPE 9 x EPE
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL
Spacegroup: P212121
Unit cell:
a: 60.54Å b: 186.25Å c: 245.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.228
Expression system: Escherichia coli DH5[alpha]

Quick links

Citations

3 review citations

Challenges and opportunities for non-antibody scaffold drugs.
Vazquez-Lombardi et al. (2015)
2 more

1 mention without citation

Tracking molecular recognition at the atomic level with a new protein scaffold based on the OB-fold.
Steemson et al. (2014)