› 4gnr

X-ray diffraction
1Å resolution

1.0 Angstrom resolution crystal structure of the branched-chain amino acid transporter substrate binding protein LivJ from Streptococcus pneumoniae str. Canada MDR_19A in complex with Isoleucine

Released:
DOI: 10.2210/pdb4gnr/pdb
PDB entry 4gnr coloured by chain, front view.
PDB entry 4gnr coloured by chain, side view.
PDB entry 4gnr coloured by chain, top view.
Source organism: Streptococcus pneumoniae str. Canada MDR_19A
Entry authors: Halavaty AS, Kudritska M, Wawrzak Z, Stogios PJ, Yim V, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125403 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine-binding protein domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 37.19 KDa
Source organism: Streptococcus pneumoniae str. Canada MDR_19A
Expression system: Escherichia coli
UniProt:
  • Canonical: J9PBT7 (Residues: 2-353; Coverage: 100%)
Sequence domains: Periplasmic binding protein
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand ILE 1 x ILE
Ligand MG 3 x MG
Ligand CL 2 x CL
1 modified residue:
Ligand MLY 1 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 37.274Å b: 72.819Å c: 57.542Å
α: 90° β: 105.1° γ: 90°
R-values:
R R work R free
0.127 0.126 0.147
Expression system: Escherichia coli

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