› 4gnv

X-ray diffraction
1.5Å resolution

Crystal structure of beta-hexosaminidase 1 from Burkholderia cenocepacia J2315 with bound N-Acetyl-D-Glucosamine

Released:
DOI: 10.2210/pdb4gnv/pdb
PDB entry 4gnv coloured by chain, front view.
PDB entry 4gnv coloured by chain, side view.
PDB entry 4gnv coloured by chain, top view.
Source organism: Burkholderia cenocepacia J2315
Entry authors: SSGCID, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-hexosaminidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 38.12 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli
UniProt:
  • Canonical: B4EA43 (Residues: 1-342; Coverage: 100%)
Gene names: BCAL1010, BCAL2860, nagZ, nagZ1, nagZ2
Sequence domains: Glycosyl hydrolase family 3 N terminal domain
Structure domains: Glycoside hydrolase, family 3, N-terminal domain

Ligands and Environments

2 bound ligands:
Ligand NAG 2 x NAG
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 48.97Å b: 89.31Å c: 67.15Å
α: 90° β: 91.73° γ: 90°
R-values:
R R work R free
0.149 0.148 0.176
Expression system: Escherichia coli

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