› 4go9

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE of the TREHALULOSE SYNTHASE MUTANT, MUTB D415N, in COMPLEX with TRIS

Released:
DOI: 10.2210/pdb4go9/pdb
PDB entry 4go9 coloured by chain, front view.
PDB entry 4go9 coloured by chain, side view.
PDB entry 4go9 coloured by chain, top view.
Source organism: Burkholderia ubonensis subsp. mesacidophila
Entry authors: Lipski A, Ravaud S, Robert X, Haser R, Aghajari N

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 557 amino acids
Theoretical weight: 63.94 KDa
Source organism: Burkholderia ubonensis subsp. mesacidophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2PS28 (Residues: 28-584; Coverage: 99%)
Gene name: mutB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21
Unit cell:
a: 64.52Å b: 84.99Å c: 123.95Å
α: 90° β: 99.53° γ: 90°
R-values:
R R work R free
0.172 0.166 0.219
Expression system: Escherichia coli

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