› 4gob

X-ray diffraction
1.53Å resolution

Low pH Crystal Structure of a reconstructed Kaede-type Red Fluorescent Protein, Least Evolved Ancestor (LEA)

Released:
DOI: 10.2210/pdb4gob/pdb
PDB entry 4gob coloured by chain, front view.
PDB entry 4gob coloured by chain, side view.
PDB entry 4gob coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Acid-base catalysis and crystal structures of a least evolved ancestral GFP-like protein undergoing green-to-red photoconversion.
Kim H, Grunkemeyer TJ, Modi C, Chen L, Fromme R, Matz MV, Wachter RM
Biochemistry 52 8048-59 (2013)
PMID: 24134825

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164511 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kaede-type Fluorescent Protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 228 amino acids
Theoretical weight: 26.43 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CR8 4 x CR8

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 82.439Å b: 100.789Å c: 120.113Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Reconstructing Ancient Proteins to Understand the Causes of Structure and Function.
Hochberg et al. (2017)
2 more

1 mention without citation

Capturing excited-state structural snapshots of evolutionary green-to-red photochromic fluorescent proteins.
Krueger et al. (2023)